Detecting viable Pseudogymnoascus destructans (Ascomycota: Pseudeurotiaceae) from walls

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Highly sensitive quantitative PCR for the detection and differentiation of Pseudogymnoascus destructans and other Pseudogymnoascus species.

White-nose syndrome is a fungal disease that has decimated bat populations across eastern North America. Identification of the etiologic agent, Pseudogymnoascus destructans (formerly Geomyces destructans), in environmental samples is essential to proposed management plans. A major challenge is the presence of closely related species, which are ubiquitous in many soils and cave sediments and oft...

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Evidence for Anti-Pseudogymnoascus destructans (Pd) Activity of Propolis

White-nose syndrome (WNS) in bats, caused by Pseudogymnoascus destructans (Pd), is a cutaneous infection that has devastated North American bat populations since 2007. At present, there is no effective method for controlling this disease. Here, we evaluated the effect of propolis against Pd in vitro. Using Sabouraud dextrose agar (SDA) medium, approximately 1.7 × 10⁷ conidia spores of the Pd st...

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Pseudogymnoascus destructans transcriptome changes during white-nose syndrome infections

White nose syndrome (WNS) is caused by the psychrophilic fungus Pseudogymnoascus destructans that can grow in the environment saprotrophically or parasitically by infecting hibernating bats. Infections are pathological in many species of North American bats, disrupting hibernation and causing mortality. To determine what fungal pathways are involved in infection of living tissue, we examined fu...

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Ectomycota Associated with Arthropods from Bat Hibernacula in Eastern Canada, with Particular Reference to Pseudogymnoascus destructans

The introduction of Pseudogymnoascus destructans (Pd) to North America, agent of white-nose syndrome in hibernating bats, has increased interest in fungi from underground habitats. While bats are assumed to be the main vector transmitting Pd cave-to-cave, the role of other fauna is unexplored. We documented the fungi associated with over-wintering arthropods in Pd-positive hibernacula, includin...

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Vitamin B2 as a virulence factor in Pseudogymnoascus destructans skin infection

Pathogenic and non-pathogenic related microorganisms differ in secondary metabolite production. Here we show that riboflavin overproduction by a fungal pathogen and its hyperaccumulation in affected host tissue exacerbates a skin infection to necrosis. In white-nose syndrome (WNS) skin lesions caused by Pseudogymnoascus destructans, maximum riboflavin concentrations reached up to 815 μg ml(-1),...

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ژورنال

عنوان ژورنال: Journal of Cave and Karst Studies

سال: 2016

ISSN: 1090-6924

DOI: 10.4311/2015mb0138